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Qu'est-ce (qui) est hybridization$36360$ - définition
![A metaphase cell positive for the ''bcr/abl'' rearrangement (associated with [[chronic myelogenous leukemia]]) using FISH. The chromosomes can be seen in blue. The chromosome that is labeled with green ''and'' red spots (upper left) is the one where the rearrangement is present.](https://commons.wikimedia.org/wiki/Special:FilePath/Bcrablmet.jpg?width=200 "A metaphase cell positive for the ''bcr/abl'' rearrangement (associated with [[chronic myelogenous leukemia]]) using FISH. The chromosomes can be seen in blue. The chromosome that is labeled with green ''and'' red spots (upper left) is the one where the rearrangement is present.")
.jpg?width=200 "Scheme of the principle of the FISH Experiment to localize a gene in the nucleus.")
.gif?width=200 "Another schematic of FISH process.")
Uh-oh, here's a new off-the-wall comedy. Time to apply the fish test.
Wikipédia
Sequencing by hybridization is a class of methods for determining the order in which nucleotides occur on a strand of DNA. Typically used for looking for small changes relative to a known DNA sequence. The binding of one strand of DNA to its complementary strand in the DNA double-helix (known as hybridization) is sensitive to even single-base mismatches when the hybrid region is short or if specialized mismatch detection proteins are present. This is exploited in a variety of ways, most notably via DNA chips or microarrays with thousands to billions of synthetic oligonucleotides found in a genome of interest plus many known variations or even all possible single-base variations.
The type of sequencing by hybridization described above has largely been displaced by other methods, including sequencing by synthesis, and sequencing by ligation (as well as pore-based methods). However hybridization of oligonucleotides is still used in some sequencing schemes, including hybridization-assisted pore-based sequencing, and reversible hybridization.
